Denitrification is the primary pathway of nitrogen (N) removal from floodplain ecosystems through conversion of N to N2 gas. Spatial and temporal heterogeneity of denitrification rates can be high, calling into question the accuracy of scaling locally measured rates across larger regions. We are using flow-through soil core incubations to assess spatial heterogeneity of denitrification potential across restored floodplain habitats (created shallow water areas, tree plantings, natural regeneration, remnant forest), and correlate variation with soil and habitat characteristics. Denitrification rates were derived from 6-12 intact soil cores collected from 30 habitats across 10 riparian wetlands. We will apply mixed effects models to investigate relationships between habitat type and age, soil moisture, distance from nearest river, and distance between soil cores within habitats, as they influence coefficients of variation and 95% confidence intervals for denitrification measurements. Results will facilitate development of strategies to account for spatial variation in regional scale denitrification estimates derived from measurements in selected local habitats. Specifically, the number and relative positions of soil cores collected for flow-through incubations can be adjusted so that sampling designs are more representative of ecological features that affect denitrification in floodplain habitats.